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By J.W. Gorrod, P. Jacob III

This ebook presents for the 1st time a unmarried finished resource of knowledge at the analytical chemistry of nicotine and comparable alkaloids. The editors have introduced jointly scientists from academia and the tobacco to explain the state of the art of the chemistry and analytical equipment for dimension of nicotine. either the scope and element of the booklet are striking. Chapters describe the historical past, pharmacology and toxicology of nicotine, the biosynthesis of nicotine and different alkaloids within the tobacco plant, the overall chemistry of nicotine and the analytical methodologies which were used to degree nicotine and comparable alkaloids in organic specimens, in tobacco and pharmaceutical items and in tobacco smoke. there's additionally a complete overview of the chemistry and toxicology of nicotine-derived nitrosamines, a huge category of tobacco cancer causing agents.

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Extra info for Analytical Determination of Nicotine and Related Compounds and their Metabolites

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Within three hours after application, radioactive material was found in the treated leaf, root and 33 Analytical Determination of Nicotine leaves younger than the treated leaf, but in not older leaves. 13% of the initial radioactivity. Even though the amount of radioactivity in the root was small compared to the total JA applied, it was yet commensurate with the increment of root JA observed after wounding. JA-conjugate pools, measured using several different hydrolysis procedures, amounted to only about 10% of the free JA pool, insufficient to account for the ten-fold increase of JA in the shoot 90 min post-wounding [100].

Chicago Press, p. 88, 1997. 103 McCloud ES, Baldwin IT. Herbivory and caterpillar regurgitants amplify the wound-induced increases in jasmonic acid but not nicotine in Nicotiana sylvestris. Planta, 1997: 203; 430-435. 43 Analytical Determination of Nicotine 104 Karban R, Baldwin IT. Induced Responses to Herbivory. Chicago: Univ. Chicago Press, 1997, p. 89. 105 Baldwin IT, Schmelz EA. Immunological "memory" in the induced accumulation of nicotine in wild tobacco. Ecology, 1996: 77; 236-246. 106 Baldwin IT, Schmelz EA, Zhang Z-P.

1990: 15; 27-38. 16 Bush LP. Physiology and biochemistry of tobacco alkaloids. Recent Adv. Tob. , 1981: 7; 75-106. 38 Biosynthesis of Nicotine and Related Compounds 17 Tiburcio AF, Galston AW. Arginine decarboxylase as the source of putrescine for tobacco alkaloids. Phytochemistry, 1986: 25; 107-110. 18 Burtin D, Michael AJ. Overexpression of arginine decarboxylase in transgenic plants. Biochem. , 1997: 325; 331-337. 19 Saunders JW, Bush LP. Nicotine biosynthetic enzyme activities in Nicotiana tabacum L.

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